NET Core Facility

Semmelweis University



Experimental Advanced Histopathology Corefacility

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List of Equipmets:

1. LEICA LED DM 3000 Microscope

Short description: The Leica DM3000 microscopes for pathology, cytology, haematology and many other applications feature a motorized nosepiece, condenser head, automated light intensity adjustment, and optional foot pedal.

Floor and Lab No: 5th floor, room 511

Contact: Zoltan Varga, email: This email address is being protected from spambots. You need JavaScript enabled to view it. , phone: +36 1 459-1500/56265

Technical assistance is provided

Required consumables (Names, CAT Numbers, Distributors, Prices, e.t.c): none

 2. LEICA CM1950 Cryostat

Short description: The Leica CM1950 basic instrument is equipped with CryoZone™ technology, UVC Disinfection, specimen orientation and AgProtect™ antimicrobial nanosilver coating.

Floor and Lab No: 5th floor, room 511

Contact: Zoltan Varga, email: This email address is being protected from spambots. You need JavaScript enabled to view it. , phone: +36 1 459-1500/56265

Technical assistance is provided

Required consumables (Names, CAT Numbers, Distributors, Prices, e.t.c):

OCT freezing medium (Leica - 14020108926), Leica blades (type 819 - 14035838925), adhesive microscope slides (Leica – 3800082E), slide box for storage (VWR – 631-1502)

 3. LEICA LMD6 laser microdissection system

Short description:

Laser Microdissection (LMD, also known as Laser Capture Microdissection or LCM) enables users to isolate specific single cells or entire areas of tissue. Powered by a unique laser design and dynamic software, Leica LMD systems allow users to easily isolate Regions of Interest (ROI) from entire areas of tissue down to single cells or even subcellular structures such as chromosomes.

LMD is typically used in genomics (DNA), transcriptomics (mRNA, miRNA), proteomics, metabolomics, and even next generation sequencing (NGS). Researchers in neurology, cancer research, plant analysis, forensics or climate research rely on this method. Furthermore, LMD is a perfect tool for live cell culture (LCC), for cloning and re-cultivation, manipulation or downstream analysis.

 Floor and Lab No: 5th floor, room 511

Contact: Zoltan Varga, email: This email address is being protected from spambots. You need JavaScript enabled to view it. , phone: +36 1 459-1500/56265

Technical assistance is provided

Required consumables (Names, CAT Numbers, Distributors, Prices, e.t.c): To be discussed. Specific slides are needed, however, their use strongly depends on the planned application/downstream analysis.

 4. Leica TP1020 Semi-enclosed Tissue Processor with vacuum and carbon filters (for formaldehyde, and for organics, Xylene standard)

Short description: The Leica TP1020 is an automatic tissue processor used for the fixation, dehydration and infiltration of histological tissue samples with fixatives, alcohol, solvents and paraffin wax.

Floor and Lab No: 5th floor, room 511

Contact: Zoltan Varga, email: This email address is being protected from spambots. You need JavaScript enabled to view it. , phone: +36 1 459-1500/56265

Technical assistance is provided.

Required consumables (Names, CAT Numbers, Distributors, Prices, e.t.c):

Xylene (Leica - 380365), ethanol (abs. – Leica - 3803686), paraffin (infiltration medium – Leica 3801340), Casettes for tissue processing (Leica – 39LC-550-4) - the details should be discussed.

 5. LEICA Arcadia H- Heated Paraffin Embedding Station

Short description: The HistoCore Arcadia H is a modern paraffin embedding station with a microprocessor control system.

Floor and Lab No: 5th floor, room 511

Contact: Zoltan Varga, email: This email address is being protected from spambots. You need JavaScript enabled to view it. , phone: +36 1 459-1500/56265

Technical assistance is provided.

Required consumables (Names, CAT Numbers, Distributors, Prices, e.t.c):

Paraffin (embedding – Leica - 3801320)

 6. LEICA Arcadia C- Cold plate with poxer cord

Short description: HistoCore Arcadia C is a cold plate holding more than 60 cassettes on its large working surface, the cold plate was designed with an environment adaptive control module to make sure the operating temperature is always stabilized at -6 °C.

Floor and Lab No: 5th floor, room 511

Contact: Zoltan Varga, email: This email address is being protected from spambots. You need JavaScript enabled to view it. , phone: 56265

Technical assistance is provided.

Required consumables (Names, CAT Numbers, Distributors, Prices, e.t.c): none

 7. LEICA EG F Electric, Heatable Forceps

Short description: The heated tips of the forceps prevent tissue from sticking to the tips and support convenient transfer of the specimen into embedding the mold.

Floor and Lab No: 5th floor, room 511

Contact: Zoltan Varga, email: This email address is being protected from spambots. You need JavaScript enabled to view it. , phone: +36 1 459-1500/56265

Technical assistance is provided.

Required consumables (Names, CAT Numbers, Distributors, Prices, e.t.c): none

8. Leica RM2245 Semi-Automated Rotary Microtome

Short description: It is the instrument of choice for clinical histology and histopathology applications, as well as for industrial quality assurance.

Floor and Lab No: 5th floor, room 511

Contact: Zoltan Varga, email: This email address is being protected from spambots. You need JavaScript enabled to view it. , phone: +36 1 459-1500/56265

Technical assistance is provided.

Required consumables (Names, CAT Numbers, Distributors, Prices, e.t.c):

Leica blades (type 819 - 14035838925), adhesive microscope slides (Leica – 3800082E), slide box for storage (VWR – 631-1502)

 9. Leica HI1210 Water bath for paraffin sections

Short description: The Leica HI1210 is a water bath with a surface that provides high thermal conductivity rates and outstanding scratch resistance due to its special plastic coating. Temperatures can be selected between ambient and 75°C.

Floor and Lab No: 5th floor, room 511

Contact: Zoltan Varga, email: This email address is being protected from spambots. You need JavaScript enabled to view it. , phone: +36 1 459-1500/56265

Technical assistance is provided.

Required consumables (Names, CAT Numbers, Distributors, Prices, e.t.c): none

 10. Leica HI1220 Flattening table

Short description: Leica HI1220 is a hot plate with high heating power and precise temperature control. The HI1220 is suitable for stretching and drying cut tissue samples in all areas of biomedical research and routine diagnostics.

Floor and Lab No: 5th floor, room 511

Contact: Zoltan Varga, email: This email address is being protected from spambots. You need JavaScript enabled to view it. , phone: +36 1 459-1500/56265

Technical assistance is provided.

Required consumables (Names, CAT Numbers, Distributors, Prices, e.t.c): none